{"id":621,"date":"2026-01-21T18:53:58","date_gmt":"2026-01-21T18:53:58","guid":{"rendered":"https:\/\/allabio.tech\/?page_id=621"},"modified":"2026-01-21T19:04:05","modified_gmt":"2026-01-21T19:04:05","slug":"immuno-pcr-elisa","status":"publish","type":"page","link":"https:\/\/allabio.tech\/index.php\/immuno-pcr-elisa\/","title":{"rendered":"Immuno PCR ELISA"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li><strong>Powerful Performance<\/strong>: Combining the specificity of ELISA with the ultra-sensitivity of digital PCR, this platform achieves detection sensitivities on par with or exceeding Olink and Somalogic, with certain targets reaching SiMoA-level performance.<\/li>\n\n\n\n<li><strong>Flexibility<\/strong>: Suitable for both biomarker discovery and single-target validation. A wide selection of assay kits is available for human and animal models, meeting the needs of diverse research applications.<\/li>\n\n\n\n<li><strong>Ease of Use<\/strong>: Utilizing magnetic bead-based technology, the workflow is straightforward and requires no dedicated instrumentation\u2014only a digital PCR system, which is readily accessible in most labs.<\/li>\n\n\n\n<li><strong>Rapid Turnaround<\/strong>: With a pre-run incubation of only 2.5 hours, single-analyte assays can be completed in as little as 4 hours.<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"466\" src=\"https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1753337370464384.jpg\" alt=\"\" class=\"wp-image-622\" srcset=\"https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1753337370464384.jpg 700w, https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1753337370464384-300x200.jpg 300w\" sizes=\"auto, (max-width: 700px) 100vw, 700px\" \/><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Powerful Performance<\/strong>: Combining the specificity of ELISA with the ultra-sensitivity of digital PCR, this platform achieves detection sensitivities on par with or exceeding Olink and Somalogic, with certain targets reaching SiMoA-level performance.<\/li>\n\n\n\n<li><strong>Flexibility<\/strong>: Suitable for both biomarker discovery and single-target validation. A wide selection of assay kits is available for human and animal models, meeting the needs of diverse research applications.<\/li>\n\n\n\n<li><strong>Ease of Use<\/strong>: Utilizing magnetic bead-based technology, the workflow is straightforward and requires no dedicated instrumentation\u2014only a digital PCR system, which is readily accessible in most labs.<\/li>\n\n\n\n<li><strong>Rapid Turnaround<\/strong>: With a pre-run incubation of only 2.5 hours, single-analyte assays can be completed in as little as 4 hours.<\/li>\n\n\n\n<li><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading has-text-align-center\"><strong>Precise, Sensitive, Convenient&nbsp;<\/strong><\/h2>\n\n\n\n<p class=\"has-text-align-center\"><strong>The Groundbreaking dIPCR ELISA Assay<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"1000\" src=\"https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1754026752173532.jpg\" alt=\"\" class=\"wp-image-623\" srcset=\"https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1754026752173532.jpg 800w, https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1754026752173532-240x300.jpg 240w, https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1754026752173532-768x960.jpg 768w\" sizes=\"auto, (max-width: 800px) 100vw, 800px\" \/><\/figure>\n\n\n\n<p>The innovative Quantitative Immuno-PCR ELISA (dIPCR ELISA) platform, powered by Turtle Technology\u2019s digital PCR systems, represents a significant advancement in protein quantification. It seamlessly merges the specificity of traditional ELISA with the exceptional sensitivity of digital PCR. Using a magnetic bead-based protocol, the system allows for accurate quantification of proteins across various sample types\u2014including serum, plasma, and cell culture supernatants\u2014with sensitivity reaching fg\/ml level. The platform supports a broad panel of human and animal models, offering robust and reliable solutions for both basic and preclinical research needs.<\/p>\n\n\n\n<p>Advantages of the dIPCR Kit over Standard ELISA:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Ultra-High Sensitivity<\/strong>: Over 1,000x more sensitive than traditional ELISA, enabling precise detection of trace proteins.<\/li>\n\n\n\n<li><strong>Minimal Sample Volume<\/strong>: Requires less than 10 \u03bcL of sample, preserving precious materials.<\/li>\n\n\n\n<li><strong>High Specificity<\/strong>: Employs a sandwich-based design for precise target recognition and reliable quantification.<\/li>\n\n\n\n<li><strong>Simple Workflow<\/strong>: Only two processing steps.<\/li>\n\n\n\n<li><strong>Efficient Process<\/strong>: Full results in under 4 hours.<\/li>\n<\/ul>\n\n\n\n<p>Simple 4-Hour Workflow Overview:<\/p>\n\n\n\n<p>1. Immune Complex Formation and Purification:<br>In a 96-well plate, samples are incubated with DAB-ONA and CAB-biotin-SA magnetic beads. When the target protein is present, DAB-ONA binds to it and attaches to CAB-biotin-SA beads via biotin-streptavidin interaction. Unbound material is removed using magnetic washing.<\/p>\n\n\n\n<p>2. PCR Amplification:<br>The immune complex is released from the beads and used as the dPCR template. PCR primers and master mix are added, and quantification is performed using a digital PCR system. Results are compared against a standard curve generated from known protein concentrations.<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"500\" src=\"https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/NexaTag_qIPCR-Principle_stacked-1.png\" alt=\"\" class=\"wp-image-625\" srcset=\"https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/NexaTag_qIPCR-Principle_stacked-1.png 600w, https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/NexaTag_qIPCR-Principle_stacked-1-300x250.png 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/figure>\n\n\n\n<p><strong>Case Study Product Specification:<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"865\" height=\"804\" src=\"https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1754027539456286.png\" alt=\"\" class=\"wp-image-626\" srcset=\"https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1754027539456286.png 865w, https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1754027539456286-300x279.png 300w, https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1754027539456286-768x714.png 768w\" sizes=\"auto, (max-width: 865px) 100vw, 865px\" \/><\/figure>\n\n\n\n<p><strong>Sensitivity vs. Olink or ELISA:<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"865\" height=\"562\" src=\"https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1757054406349828.png\" alt=\"\" class=\"wp-image-627\" srcset=\"https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1757054406349828.png 865w, https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1757054406349828-300x195.png 300w, https:\/\/allabio.tech\/wp-content\/uploads\/2026\/01\/1757054406349828-768x499.png 768w\" sizes=\"auto, (max-width: 865px) 100vw, 865px\" \/><\/figure>\n\n\n\n<p><strong>Enquiry 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